HRBR Automatic Multi-Plate Bioreactor (AMPBR) System
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The AMPBR is based on our HRBR technology for adherent cell cultures using 2D multi-tray culture vessel. The novel automatic multi-plate bioreactor (AMPBR) system is similar to our automatic multi-tray bioreactor (AMTBR) system except the vessel comprising a single closed vessel filled with a module of multiple plates. AMTBR comprises multiple trays stacked together and forms multiple compartments communicated with common manifolds. AMPBR has only one compartment. The oxygenation of this system relies primarily on the performance of Roller Bottle Principle.
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How Does It Work?• Use of HRBR technology and a supporting container which holds the multi-plate vessel to serve as an incubator. Also performs all culture processes in one closed system
• Application of active gassing to promote the gas exchange in the gas phase. • Performance of the roller bottle principle for the most efficient oxygenation. |
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The below tables illustrates the major differences between the AMTBR and the AMCF.
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System Features |
Conventional multi-layer 40 tray vessel Cell Factory or Cellstack |
Conventional multi-layer 40 tray vessel Cell Factory, with AMCF system (auto-manipulator) |
Conventional multi-layer 40 tray vessel Cell Factory, with AMTBR1 |
Modified multi-tray vessel I with AMTBR1 |
Modified multi-tray vessel II (w/o turncock) with AMTBR2 |
Modified multi-tray vessel II (w turncock) with AMTBR2 |
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Operation |
All manual |
Semi-automated |
Total automation |
Total automation |
Total automation |
Total automation |
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Controllability |
Minimal |
Minimal |
Maximum |
Maximum |
Maximum |
Maximum |
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Vessel Size for Equiv. Surface Area (25,280cm3/vessel) |
47.5 |
47.5 |
47.5 |
15.84 |
15.84 |
7.92 |
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Capital Cost |
$100k |
$700k+ |
Less than $100k |
Less than $80k |
Less than $70k |
Less than $50k |
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Labor Intensive? |
Yes |
Yes |
No |
No |
No |
No |
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Closed System? |
No |
No |
Yes |
Yes |
Yes |
Yes |
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Gassing |
Passive |
Passive |
Active |
Active |
Active |
Active |
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Platform |
n/a |
n/a |
See-saw |
See-saw |
Fixed |
Fixed |
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Rotating Action |
3D |
3D |
3D |
3D |
2D |
2D |
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Culture Method |
Static |
Static |
Static |
Static |
Static |
Dynamic |
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Function |
Limited static culture only |
Limited static culture only |
Unlimited bioreactor function |
Unlimited bioreactor function |
Unlimited bioreactor function |
Unlimited bioreactor function |
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Separate CO2 Incubator? |
Yes |
Yes |
No |
No |
No |
No |
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Seeding |
Manual |
Semi-Auto |
Auto |
Auto |
Auto |
Auto |
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Medium Exchange (Filling/Emptying) |
Manual |
Semi-Auto |
Auto |
Auto |
Auto |
Auto |
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Infection / Transfection |
Manual |
Semi-Auto |
Auto |
Auto |
Auto |
Auto |
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Cell Detachment |
Manual |
Semi-Auto |
Auto |
Auto |
Auto |
Auto |
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Sampling |
Manual |
Manual |
Auto |
Auto |
Auto |
Auto |
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Harvesting |
Manual |
Semi-Auto |
Auto |
Auto |
Auto |
Auto |
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Model No. |
Description |
Vessel Type |
No. of Trays |
No. of Vessels |
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HRBR130 |
Automatic Multi-Tray Bioreactor 1 (AMTBR1) |
Cell Factory* |
2, 4, 10, & 40 |
1,2,4 |
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MTV1** |
n/a |
1,2,4 |
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HRBR131 |
Automatic Multi-tray Bioreactor 2 (AMBR2) |
MTV2** |
n/a |
1,2,4 |
*or the equivalent i.e. Cellstack
** not available at this time
The
size of the AMTBR 1 & 2 is dependent on the type of vessel, the number of
trays & vessels.
