HRBR for Suspension and Adherent/MICROCARRIER Cultures
The HRBR for Suspension and Adherent/Microcarrier cultures are the first of its kind based on our patented HRBR technology for suspension and microcarrier cultures. This new bioreactor possesses all of the benefits of the conventional stirred bioreactor system while resolving their weaknesses. This HRBR requires no impeller agitation, and cultures at low rocking rate (2-3 rpm) but at an angle of 180 degrees, yielding effective mixing and extremely low shear stress. The low rocking rate, or tumbling effect eliminates the limitation of microcarrier suspension by agitation. Owing to the below features, the HRBR has established itself as a distinct innovative bioprocessing technology for the mass production of bioproducts.
Features
No Agitator, Low Rocking Rate <5rpm
Extremely low shear stress for all scales
Easy to: scale up; convert to single use; construct and operate
Large working capacity (>75%)
High microcarrier loading capacity (50-100 g/L)
No foaming issues
how does it work?
Use of a special baffle design: a large baffle is sealed to all contact surfaces assuring little or no leakage of fluid through the baffle contact points and only over the top of it as the vessel rocks. This assures potent mixing with very little shear force. Every rock of the vessel yields 2 complete "turn-overs" of the entire fluid contents of the vessel. The low rocking rate of only <5 rpm therefore results in effective mixing of the microcarriers regardless of how heavy the load.
Two sets of gassing ports alternately controlled to assure that gas passage is in the gas phase for surface aeration as the liquid contents falls over the baffle. The surface aeration avoids foaming concerns.
