MA-100/150 Mechanically-Agitated Bioreactor
MA-100 Mechanically-Agitated Bioreactor
MA-150 Mechanically-Agitated Bioreactor
SPECIFICATIONS
MA-100: microbial culture; MA-150: cell culture
Size: total volume: 1, 4, 7, 14 L; working volume is approximately 35-80% of total volume depending on operating conditions and internal configuration.
Vessel: glass size (total volume): 1, 4, 7, 14 L. Vessels are interchangeable for systems using the same control console.
Glass vessel includes heating/cooling S/S jacketed bottom (std) or with internal heating/cooling coil (optional) that utilizes water circulation system (for lighter weight) and is good for both microbial and cell cultures. Also optionally available with glass vessel with only heating pad and without S/S cooling jacket for light weight - good for only cell culture.
Agitation speed: 0-1500/0-1000/0-600 rpm (impeller ratio: 1/2) for MA-100; 0-100/0-200 rpm for MA- 150
Head plate: multiple sampling/feeding ports, 12mm ports for pH/DO probes, 8mm port for temperature probe; equipped with high efficient air condenser; handles on each end for easy transportation; mounted with agitation unit.
Power: 115v or 220v /50-60Hz
Dry weight: 70 Kg (approximate but varies with vessel size)
Autoclave sterilization or [OPTIONAL] semi-automated steam sterilization in place using a S/S cylindrical cover sealed to the S/S jacketed base and control the sterilization temperature by controlling the inlet of steam generated from a steam generator such as a small lab autoclave. In general some lab autoclaves can accommodate 5L bioreactors with height of <70 cm.
DO control range: maintain DO above the setpoint (controlling in cascade Agitation, AIR ,O2/ and Head pressure)#
# To use air to control air flow rate requiring a mass flow controller.
#It is most efficient to use O2 and head pressure to control DO. There is no need to use a mass flow controller but requiring an additional rotameter with a on/off control valve.(optional)
#Air flow rate can be controlled manually and accurately by a rotameter .
#To control the head pressure of vessel requires a pressure control module (optional).
SPECIAL FEATURES
All vessels larger than 1L can operate at medium volume below 1L and as low as 700 ml using our special agitator. This is specially useful for cell culture studies which the cost of each experiment is relatively expensive in terms of the medium and culture propagation compared to the microbial cultures
The system contains an easy programmable software to alter the control set points including agitation speed, temperature, pH and DO as well as various feeding/flow rates in any preprogrammed patterns during the culture process.
The system is capable of operating at very low and accurate agitation speed as low as 5 rpm which is specially beneficial microcarrier culture during the cell attachment process.
The system has a special agitator ideally for microcarrier culture where a gentle and low speed agitation are required.